Abstract: Oil was extracted from the seed of Allanblackia floribunda using hot water floatation method. The oil was characterized and used for production of biodiesel through transesterification process. Evaluation of the properties of the biodiesel obtained was done. Physicochemical properties of the oil were measured and the results obtained show that the oil is edible and can also be used for industrial purposes. The viscosity of oil was 30 Cm2S-1, density 914 kg/m3, smoke point 210 ˚C, flash point 270 ˚C, cloud point 25 ˚C and refractive index of 1.464 nD40. Transesterification was carried out at a temperature of 60 °C for 60 minutes with 6:1 mole ratio of the methanol to oil and 1 gram of KOH as catalyst.