Abstract: The success of insemination programme depends on the appropriate management of semen collection, storage and use. According to some reports, the highest numbers of successful AIs were done in Central Province (CP) and the lowest were recorded in Eastern Province and Northern Province. So far there are no studies related to semen quality of bull which belongs to AI centre at Thirunelvely. The semen of Jersey was collected by means of artificial vagina and stored at 4-8°C. General examination was evaluated to assess volume, colour, opacity and pH. Fresh semen (0 h) and stored semen (24 h to 72 h) were compared by progressive individual motility and sperm count. Sperm count was evaluated by hemocytometer. Viability was determined by using 1% eosin. Repeated measures analysis of variance (ANOVA) with Dunnett’s post test was performed using prism 5.04 to compare the viability of semen from 0 h to 72 h during storage. Viability decreased significantly (ANOVA P<0.05) in a time dependent manner from 0 h to 72 h during storage at 4-8°C. The percentage of viable sperms and sperm velocity gradually decreased when compared to control 0 h (93.17± 1.322%, 23.33 ± 1.419 μm/s), with 24 h (85.50 ± 0.8892 %, 13.33± 0.7698 μm/s), 48 h (79.22 ± 0.7731, 1.778 ± 0.2383 μm/s) and 72 h (69.17 ± 1.360 %, 0.817 ± 0.271μm/s). Our preliminary studies suggested that the fresh semen is better than the stored semen for higher successful rate of insemination in NP of Sri Lanka.