International Journal of Scientific and Research Publications

IJSRP, Volume 2, Issue 12, December 2012 Edition [ISSN 2250-3153]


Liquid Chromatographic Technique for Stability Indicating Analytical Method Development and Validation of Salicylic Acid and Tolnaftate in Pharmaceutical Ointment by High Performance
      Safeena Sheikh, Suhail Asghar, Showkat Ahmad Patni
Abstract: A stability indicating high performance liquid chromatography (HPLC) method is developed for the quantification of salicylic acid (SA) and Tolnaftate (TF) in combined pharmaceutical ointment base formulations. The separation was performed on a Merck” C-18 column with the mobile phase consisting of Acetonitrile: Methanol: Water (50:20:30v/v) at flow rate 1.5ml/min. Both the drugs were resolved successfully with retention time 1.318 and 8.805minute when detection was carried out at UV 245nm.The overall retention time of analytes were 10.0minutes.The method was validated with respect to linearity, precision, accuracy and recovery. The relative standard deviation for six replicate measurements of SA and TF were 0.259% and 0.240% respectively. Total recoveries of analytes were 100.56, 100.63, 100.58% and 100.23, 100.73, 100.22% of SA and TF respectively when examine over the range of 80, 100, and 120% of added drugs in placebo.

Reference this Research Paper (copy & paste below code):

Safeena Sheikh, Suhail Asghar, Showkat Ahmad Patni (2018); Liquid Chromatographic Technique for Stability Indicating Analytical Method Development and Validation of Salicylic Acid and Tolnaftate in Pharmaceutical Ointment by High Performance; Int J Sci Res Publ 2(12) (ISSN: 2250-3153). http://www.ijsrp.org/research-paper-1212.php?rp=P12481
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