Abstract: A validated, specific, stability indicating reverse phase liquid chromatographic method has been developed for simultaneous quantitative analysis of Phenylephrine HCl, Lignocaine HCl and Betamethasone valerate in pharmaceutical ointment base products. The method was optimized by analysis of the samples and sample solutions spiked with each analyte for recovery study. Good resolution between the analytes was achieved in formulation and combined standards on Merck’ C18 (250mm X 4.6mm, 5µ) column with mobile phase constituted of phosphate buffer (0.01M) and acetonitrile (46: 54% v/v) further the pH of the mobile phase was adjusted to pH=7.0(±0.05) with triethylamine. Detection was performed at 270nm.The method was validated in accordance with ICH guidelines and validation data showed that the assay is sensitive, specific and reproducible for the simultaneous estimation of Phenylephrine HCl, Lignocaine HCl and Betamethasone Valerate in the presence of other pharmaceutical excipient.