Abstract: This study was designed to highlight the food sovereignty practices of the respondents in the context of African spirituality of smallholder farmers in Matayos Sub-County of Busia County Kenya, and its impact on their food security status. Quantitave survey data was collected from 399 households’ using self-administered questionnaires, whereas qualitative data was derived from focus group discussions and case studies and reported verbertim. The results indicated that being custodians of the culture and traditions in African spirituality through the staple food crop value chain, the elders cared for the environment and passed the knowledge to younger generations in various food sovereignty practices through both learned modern and traditional farm practices including land preparation, planting, harvesting, post-harvest handling, processing and utilization of food for abetter food security of the farm households.