Abstract: Introduction: This study captures the perspectives of stakeholders on optimization of Nigeria vaccine supply chain with 3D printing technology. Temperature excursion, cold chain maintenance and the attendant issues of maintaining vaccine integrity across the supply chain network till the last mile remain a critical bottleneck to immunization and vaccine coverage. This study aimed at taking a pragmatic approach to assessing the awareness, acceptability, feasibility, and preparedness of the Nigerian supply chain professionals to take up the innovation of 3D technology to optimize the vaccine supply chain and immunization coverage in the Nigeria context. Internet-based questionnaire (Google Form) was used to reach different public health supply chain professionals in Nigeria.