Abstract: Background: The increase in the price of fossil fuel, strict government regulations on exhaust emissions and future depletion of worldwide petroleum reserves trigger studies to look for alternative fuels. Bio-ethanol from different kinds of biomass is one way to reduce both consumption of crude oil and environmental pollution. Although extensive efforts have been put in place to evaluate the potential of microalgae as a biofuels feedstock during the past 4–5 decades but there is currently limited information on the state of Microalgae biomass conversion to ethanol, this study aimed at harnessing the potentials of microalgae as third generation biomass for bioethanol production.