Abstract: Objectives:To assess nurses knowledge regarding the facilitated tucking position during venipuncture, and assess the effectiveness of education program among nurses by comparing the pre- and post-test knowledge scores regarding the facilitated tucking position, and it is also, to determine the relationship between the pre- and post-test knowledge scores and the demographic variable of nurses by a Non-probability (purposive sample) of (25) all of them day shift working in Neonatal intensive care units (NICU) at AL-Batool teaching hospital.