Abstract: Environmental and hospital-sampling allowed us to isolate and identify one hundred of Pseudomonas aeruginosa (P. aeruginosa) strains. The study of the degradation capacity of alkanes has shown that almost all isolates grew in the presence of long chain n-alkanes, while no growth was recorded in the presence of short chain n-alkanes, isoalkanes or cycloalkanes. The kinetics of growth in the presence of hexadecane, as a sole carbon source, enabled recording various optical densities (OD) depending on the strain of P. aeruginosa. The polymerase chain reaction (PCR) in the presence of ERIC (Enterobacterial repetitive intergenic consensus) primers has shown genetic diversity within isolates.