Abstract: Pangasius hypophtalmus bone is a waste that can be used as raw material for gelatin. Gelatin is produced by partial hydrolysis of collagen in fish bones. This research aims to find out the best extraction time that produces high strength and high viscosity gel. The extraction time used was 5, 6 and 7 hours. The best gelatin results were obtained at 7 hours extraction time of stretch gel (173.3 bloom), viscosity (4.05) and amino acid glycine content (19.95%), proline (10.88%), alanine (8.05% )