Abstract: Antimicrobial use in animal’s production has led to the emergence of resistant of Escherichia coli. A study on antibiotic resistance and plasmid profile of E. coli from faecal samples of Cattle from Keffi Abattoir, Nigeria was carried out. A total of 50 faecal samples were collected and E. coli was isolated and identified using standard microbiological method. The antibiotic resistance was determined in complianc with the clinical and laboratory standard institute (CLSI) method while the plasmid DNA of the multiple antibiotic resistance isolates were determined using stardand molecular method.