Abstract: Lymphadenitis is an infection of the lymph nodes. This can be caused by infection with Mycobacterium tuberculosis or Atypical Mycobacterium. Infection due to Atypical Mycobacterium is increasing in incidence worldwide, but it is difficult to overcome infections caused by Atypical Mycobacterium. The clinical symptoms of this infection are often the same as tuberculosis so often diagnosed with tuberculosis. Objective: To find out whether there is Atypical Mycobacterium in a fine needle aspiration biopsy sedan confirmed by examination of polymerase chain reaction (PCR) in Indonesia, especially in Medan.