Abstract: The present study an attempt was made to determine the effect of the mutagen (EMS) on seed germination in blackgram mutation breeding program. The blackgram accession IC436585 was collected from NBPGR regional centre, Hyderabad was used in the present study. The results showed that the percentage (%) of seed germination in black gram, subjected to treatment with different concentrations of EMS is less than that of their Control. It is clearly indicates that the mutagen (EMS) have clearly exerted an inhibitory effect on seed germination. Percent of seed germination has decreased with an increase in concentration or dose of the mutagens (EMS). The results also revealed that the black gram cultivar was more sensitive to the mutagens EMS. The results also showed that the shoot and root length were decreased with an increase in concentration or dose of the mutagens (EMS). It may concluded that the blackgram genotype IC 436585 may be germinated up to 0.3% EMS concentration to create variability in blackgram. Further variable lines are useful for crop improvement programe.