Abstract: Edible Bird Nest Swiftlets naturally inhabits the lime stone caves of South East Asia, but recently swiftlet ranching in house farms has become popular. These swiftlets are of great commercial importance because of their unique nest produced wholly from the pair of salivary gland and highly valued for their nutritional, medicinal and aphrodisiac properties. The study elucidates mitochondrial variability information of A. fuciphagus by analysing two mtDNA genes (cytb and ND5).