Abstract: Diabetes mellitus is a chronic metabolic disease that is associated with abnormal metabolism of carbohydrates, proteins and fat. It is usually associated with excretion of excess sweet urine known as glycosuria. This study investigated the anti-diabetic activity of seed milk extract in Alloxan-induced diabetic rats. The seed milk extract at a concentration of 100,200, 300 and 400 mg/kg body weight were orally administered to alloxan-induced diabetic rats for a period of fifteen (15) days. The oral glucose tolerance test was also carried out using animal experimental method.