Abstract: The potato virus Y (PVY) is disseminated globally and is one of the most devastating viruses with respect to yield and quality deteriorations in the potato (Solanum tuberosum L.). PVY caused crop losses may reach even 80%. The use of extreme resistant cultivars is assumed to be the most effective and sustainable long term way of reducing crop losses associated with PVY virus. To cut down this impingement, there is an immense interest in obtaining genotypes carrying the Ryadg and Rysto genes that provide extreme resistance to this virus. These two genes derived from Solanum tuberosumssp S. andigena as well as S. stoloniferum, respectively, are reside on different chromosomes. Introgression of these genes could be facilitated using the Ryadg specific RYSC3 and the Rysto specific ST1 SCAR (Sequence Characterized Amplified Region) markers for marker - assisted selection (MAS). The objective of the present study was to evaluate different potato genotypes with these SCAR markers for the presence or absence of Ryadg and /or Rysto PVY extreme resistance genes. Of a total of 13 evaluated potato breeding lines three genotypes (Cmm16, 96.367 and FH97. 029. 02) revealed resistance to the virus (PVY), and it was found that they are carrying both the RYSC3 and ST1 markers; whereas the RYSC3 marker could be detected in all tested genotypes. Here, we declared this highly effective (100%) detection of the Ryadg gene confirmed that the marker is appropriate in MAS of potato genotypes with extreme PVY resistance. Our results indicate that marker assisted selection for these two genes could be effectively used in potato breeding for the production of PVY extreme resistance genotypes, even in hetero-multiplex condition.