Abstract: Phototoxic and photoallergic reactions represent skin reactions to the sun, in the presence of photoactive chemicals applied on the skin or taken systemically. They have a highly polymorphic clinical presentation – photo contact urticaria, eczema on sun-exposed areas sometimes with erythema multiforme, exaggerated sunburn, linear phytophotodermatitis, pseudoporphyria, photoonycholysis, dyschromia, and lupus erythematosus. Also, skin cancers are increasingly associated with exposure to photoactive chemicals. There is a geographical and timely variation in the responsible agents, but they are mostly furocumarins from plants, UV filters in sunscreens and cosmetics, and drugs (NSAIDs, antimicrobials, phenothiazines, amiodarone, etc.) Three drugs like Chloramphenicol, Norflox, and Kucil(Fluorouracil) were tested for Phototoxic effect procured from local market and check the ph and absorption spectra and note and solubility of test drug was tried out through different solvents viz. DW (Distill water), petroleum ether, ethanol, DMSO. The Phototoxic activity was studied against the bacterial culture Escherichia coli (DH5 α).The Results of this study revealed that these drugs seem to be phototoxic effect on Microbial culture.