Abstract: Biopolymer producing bacterial species (Bacillus and Pseudomonas )were isolated from rhizosphere soil of Jatropha curcas by using standard morphological, cultural and biochemical characteristics.PHB production by bacterial sps were screened by Sudan black B staining method. The main aim of the present study to production of biopolymers using crude glycerol is a byproduct of biodiesel, act as a sole carbon source for PHB producing microorganisms, under the limitation of nitrogen and phosphate . Ammonium molybdate and ammonium sulphate were used in nitrogen deficient medium instead of ammonium chloride. Maximum biopolymer production were noted in Bacillus spp13.3 gm/100ml.Pseudomonas spp yielded 11.2 gm/100 ml.