Abstract: Electricity generation from microbial fuel cell (MFC) using cow dung and cow urine as fuel was investigated in this study. The electrodes used in MFC were combinations of copper, brass and magnesium sheets of 0.2 mm thickness and having 6”X 6” dimension. Comparative studies resulted from using cow dung as fuel in two chamber design of MFC under aerobic and anaerobic conditions. Studies were carried out using cow urine as fuel in six chamber design of MFC under anaerobic condition. Dual chamber design and six chamber design gave an average output of 1.1 volt and 4.2 volt respectively.