Abstract: Mortierella ramanniana is known as a gamma linolenic acid as an gamma linolenic acid producing oleaginous fungus. Enrichment of GLA from the fungal lipids by a urea inclusion method was studied. Most of the saturated and monosaturated, 93.0% and 84.6%, respectively were removed by forming urea inclusion compounds. GLA was concentrated after urea inclusion. Its content in total fatty acids increased 6.2 folds and recovery of reached 57.1% with a recovery of 90%.