Abstract: Chronic Renal Failure (CRF) patients are at risk of cardiovascular diseases due to the elevation of various forms of lipids. Many a time CRF patients live on hemodialysis on regular basis. Present study was done to know whether hemodialysis has any impact on the lipid profile of the CRF patients. Study were divided into 3 groups, Group-I: healthy controls (30), Group-II: CRF patients who never undergone hemodialysis (30) and Group-III: CRF patients on hemodialysis for more than 6 months (30). We obtained serum samples from patients in the morning after an overnight fast and were analyzed for total cholesterol (TC), triglycerides (TGs), HDL, LDL and VLDL.