Abstract: Calendula Officinalis which belongs to the family Asteraceae, and most commonly known as ‘pot marigold’, is used medicinally in different forms like infusion, tinctures, liquid extracts, creams or ointment. Aim: The aim of present was to evaluate the anti-microbial effect of different concentrations of calendula extract (Calendula officinalis) on periodontal pathogens. Material and Methods: The study was conducted on twenty five patients having chronic gingivitis and twenty five patients having chronic periodontitis above the age of 18 years were enrolled in the study with the written informed consent.