Abstract: In this research work, wound dressing materials were prepared as composite membranes from chitosan, sodium alginate and calcium chloride by autoclaving method. The important parameters for wound dressing materials are tensile strength, elongation at break, tear strength, antibacterial activities and wound healing activity. Moreover biodegradability of composite membrane is one of the important parameters for reduction environmental pollution. This research work concerns with the preparation, characterization and application of biodegradable modified chitosan composite membranes (MCM) for wound dressing materials.