Abstract: Infant food products were collected from supermarkets in Sri Lanka and investigated for contamination by fungi. The objective of this study was to develop a novel DNA extraction procedure and a PCR assay for the detection of fungal presence which leads to contamination of infant foods. Mycotoxins are secondary metabolites of many phytopathogenic and food spoilage fungi. The presence of mycotoxins in the food chain is of high concern for human health due to the ability of these compounds to induce severe toxic effects.