Abstract: Iris recognition is a biometric technology to identify humans by capturing and analyzing the distinctive patterns of iris within the human eye. This paper involves developing an ‘open-source’ iris recognition system so as to verify each individuality of the human iris and conjointly its performance as a biometric.To determine the recognition performance of the system databases of grey-scale eye images are used. The development tool to be used is MATLAB, which emphasis only on performing recognition, and not on capturing of an eye image using hardware.