Abstract: This research have assessed nitric oxide (NO) levels in hemorrhoids. The study was conducted a cross sectional study to look at the profile of free radical nitric oxide (NO) with ELISA method in 80 hemorrhoids patients at Surgery Department of Hasanuddin University Faculty of Medicine, Wahidin Sudirohusodo Hospital and other network hospitals. Patients are aggregated and grouped by age, sex, criterion of smokers, drinking criteria, occupation, and diet. Nitric Oxide levels are elevated in grade III and IV hemorrhoids.