Abstract: Background: Acquired hospital infection most of factors responsible for morbidity and mortality ,especially in critical care units , it is one of most dangerous units because they are crowded with patients who are much closer to each other as well as the most frequently penetrating procedures of the skin and mucous membrane beside that the length of patient hospitalization and treatment this increased susceptibility to infection. The study aims: to evaluate the nurse’s practice for the sterile technique in critical care units concerning hand washing, uniform, gloves, gown, mask, instruments and equipment, Peripheral Venous Catheter, IV fluids and medication, medical waste management and sharp disposal and to find out relationship between the nurses practices and nurses demographic characteristics.