Abstract: Medicinal plants are big source of information for wide variety of chemical constituents which could be developed as drug with precise selectivity. Achyranthes aspera Linn is one of the important medicinal plants having many therapeutic uses. The present study deals with preliminary phytochemical screening and TLC investigation of Achyranthes aspera Linn. In qualitative analysis, the phytochemical compounds such as steroids, triterpenoids, sugars, alkaloids, phenolic compounds, flavonoids were screened in root methanolic extract by using standard methods. Phytochemical screening of plant revealed the presence of alkaloids, flavonoids steroids reducing sugar, glycosides and Terpenoids. The present study revealed that Achyranthes aspera roots are important source of many therapeutically and pharmacologically active constituents.