Abstract: The Aim and Objective of the present study was to formulate and evaluate the novel ocular Inserts of Diclofenac amino acid conjugate using different polymers such as HPMC, Eudragit L100 at various concentrations and combinations using dibutylphthalate as plasticizer. The ocuserts were prepared by using solvent casting technique. The main aim was to enhance the trancorneal permeation of diclofenac sodium using different amino acids.Ex vivo trancorneal permeation was studied across goat cornea. The study revealed that Diclofenac sodium and amino acid conjugate ocular inserts had greater permeation than Diclofenac sodium ocular inserts.Effect of concentration of Amino acids on the permeation of Diclofenac sodium was also studied and it was found that on increasing the concentration of amino Acid ,permeation of Diclofenac Sodium increases.Diclofenac sodium and Lysine conjugate produced maximum ocular bioavailability. Formulations(F1) of Diclofenac sodium was prepared and compared, with different formulations ( F2,F3,F4) of Diclofenac sodium and Amino Acid Conjugates and it was found that F4 has maximum ocular availability.. The optimized formulation was subjected to stability studies as per ICH guidelines.