Abstract: The present study was carried out to assess the physico-chemical properties of Loktak lake at five selected villages– three lakeshore villages i.e. Phoubakchao, Laphupat Tera, Nongmaikhong and two island villages-Ithing and Karang. A total of eleven parameters were monitored for a period of one year to assess the standard of water quality of Loktak lake with reference to its status of pollution. It was observed that air temperature ranged between 11-33°C, water temperature between16-32°C, pH ranged between 6.05-9.10, Dissolved oxygen between 4.05-14.18 mg/l, Biochemical oxygen demand between 1.51-10.65 mg/l, Free CO2 between 0-35 mg/l, Total dissolved solids between 50-150 ppm, Phenolphthalein Alkalinity between 0-20 mg/l, Total Alkalinity between 35-90 mg/l, Conductivity between 90-220 μS/cm and Transparency between 29-162 cm.