Abstract: E-learning has influenced every sector of the society and can be used as a tool to improve the quality of education for the society and manpower. A study was undertaken to develop an e-content package related to the topic DNA replication included in Botany for XII standard students of Biology and to compare the effectiveness of this package with conventional teaching methods. The e-content has been developed for learning the concept: DNA replication using e-content package from XII standard botany text book prescribed by Tamil Nadu Text Book Society. A pre-test, post-test single group experimental design was adopted. A sample of 20 XII standard students were exposed to different treatments namely e-content package and conventional method of teaching. The control and the experimental groups were assessed through pre and post tests and their achievement scores were compiled and analyzed using appropriate statistical procedure.