Abstract: To investigate the removal efficiency of Methylene blue (MB) by using TiO2 with second metal (M) such as Cu, Al, Mg, Fe, Tb, La and Ce as catalyst which were prepared by im-pregnation method in amount of 2% M/TiO2. The catalyst ratio that used in this reaction was 2 g/L, pH 5, temperature 30˚C and 100 min in reaction time. The most effective catalyst was 2%Cu/ TiO2. Then the preparations of 2%Cu/ TiO2 were compared; im-pregnation and sol-gel process. The study found that 2%Cu/ TiO2 prepared by the im-pregnation method performed better activity. Therefore, we selected to prepare the catalyst by the im-pregnation method to study the amount of