IJSRP, Volume 3, Issue 6, June 2013 Edition [ISSN 2250-3153]
Chitra S, Krithika M.V
The present study was to investigate the possible chemopreventive activity of green tea polyphenol, (-)-Epigallocatechin-3-gallate (EGCG) on laryngeal cancer cells. Cell viability was evaluated using MTT assay. Activity of superoxide dismutase, cytotoxicity was assessed using lactate dehydrogenase and clonogenic assay was done using crystal violet stain. Apoptotic cell death was confirmed by DNA fragmentation and cell cycle arrest was analyzed by flow cytometry using propedium iodide. The obtained results showed that the cell viability of Hep2 cells was very much reduced in EGCG treated cells. Significant increase in SOD activity (p<0.001) and a significant decrease in LDH activity (p<0.001) was noticed in EGCG treated group. Clonogenic assay and continuous shear in DNA fragmentation was observed in EGCG treated group.