Abstract: To improve the antimicrobial compound productivity of Streptomyces sp KMA08 by optimizing its physical and chemical parameters. Streptomyces sp KMA08 was isolated from mangrove soil of pitchavaram and was screened for its antimicrobial activity by using agar well diffusion method. To improve the production of antimicrobial compound the medium composition and physical parameters were optimized and its productivity was studied against Staphylococcus aureus Escherichia coli and Candida albicans. Optimum growth of mycelium and antimicrobial compound production occurred at pH 7.2, agitation 180 rpm and temperature 30 ˚ C with glucose 10g/L, soyabean meal 2.5g/L, K2HPO4 2g/L, MgSO4. The optimization of cultural conditions proposed in this paper has effetely improved the antimicrobial compound productivity of Streptomyces sp KMA 08