Abstract: Electroporation, a biophysical effect finds immense significance in the fields of medical applications. Used as a method of transfection, it can be employed to find cure for three dangerous and life threatening medical conditions like cancer, decrease in insulin production and autoimmune diseases. Electrical apparatus used for these treatments are generally based on dc voltage pulses, which due to their inefficiency in producing desired output, have paved ways for electrical apparatus that use ac pulses. Therefore, a high voltage linear amplifier design using vacuum tube valves has been referred to, for the production of the same.