Abstract: This paper reported the outcomes of a facile and active Gold nanoparticle synthesis approach based on aqueous solution of gum ghatti and HAuCl4, without addition of any hazardous chemical for the reduction and capping agent. However, formed effective stabilization of Gold nanoparticles in gum ghatti (AuNPGT) with a fine size and average diameter was 5±2 nm. The successful synthesis of AuNPGT was confirmed by UV–visible spectroscopy, Fourier transform infrared spectroscopy, X-ray powder diffraction, Transmission electron microscopy, EDS and DLS.