Abstract: To find out the effect of training on selected biochemical variables of urban employee of district Budgam. For the purpose of this study 50 urban employees from various departments were selected randomly from the district Budgam of Jammu And Kashmir State aged between 35 to 45 years. They were divided in to two equally groups (experiment and control). The experiment group containing 25 subjects underwent gym training programme. controlled group comprised of 25 subjects did not received any kind of gym training programme for the same period of time. After completing the eight weeks gym training programme (experiment) post test was conducted in the same way by testing the blood samples of the subjects. The data were collected on selected biochemical variables (determination of haemoglobin, serum urea, serum uric acid, total cholesterol, triglycerol, HDL-C and LDL-C.) The entire tests were done at IRAM CLINICAL LAB BUDGAM to get better results. The result concluded that the regular gym training programme contributes to the improve the selected biochemical variables indicating the good health status of urban employees.