Abstract: Platelet-rich plasma (PRP) is a platelet concentrate that has been used widely to accelerate soft tissue and hard-tissue healing. Platelet-rich fibrin (PRF) was first described by Choukroun et al. in France. It has been referred to as a second-generation platelet concentrate, which has been shown to have several advantages over traditionally prepared PRP. Two patients reported to the Department of Conservative Dentistry and Endodontics in North Bengal Dental College & Hospital with established pulpitis in molar. Teeth had carious pulp exposure, with a history of lingering pain. After isolation, caries removal and pulp exposure, pulpotomy with PRF was performed and a permanent restoration was placed immediately. At the first recall (+1 day), no postoperative pain was reported. At 3, 6 and 12 months recall, the tooth responded positively to pulp sensibility tests, and radiographic examination revealed a normal periodontal ligament space. Positive results of these cases imply the need for more studies with larger sample sizes and a longer recall period to justify the use of this novel material for the treatment of pulpitis in human permanent molar teeth.