Abstract: Pectinase producing microorganisms were isolated from pectin rich sites using selective isolation technique. These studies deal with screening the pectinase enzyme producing microorganisms. Best pectinolytic activity, as indicated by the diameter of clear, hydrolyzed zones on the medium plates containing commercial citrus pectin as sole carbon source. The strains of Penicillium spp and Aspergillus sps have good prospect for Pectinase production microorganisms based on the zone formation.