Abstract: The study is interventional in nature because the training has been done as an intervention. The study was done to find out the impact of training on knowledge level of the hospital staff who is dealing with biomedical waste on day to day basis. The study was conducted on 184 staff members during July – Sept 2012 in multispecialty tertiary care hospital. The survey form was prepared and was applied to all participants in person before and after the training was conducted. The training programme on biomedical waste management was for total 60 hours of which 40 hours were class room lectures and 20 hours practice sessions. the Methods used in the analysis of data were chi-square and t-tests. Of total study participants 71.7% (132) were female while 28.2%(52) were male. nursing staff constituted 54.3% (100), medical staff 20.1% (37), house keeping 17.3% (32) while general management 8.1% (15). a significant statistical difference (pretraining and post training) was found among these staff members who have received training in biomedical waste management which is evident from the raised level of knowledge and awareness about biomedical waste management. The safe management of biomedical waste is of paramount importance for the hospital staff, patients as well as community population. Hospital staff is responsible for safe disposal of waste and that can be reinforce with the help of structured training programme.