Abstract: Rearing barramundi larvae using natural food is considered ineffective, so it needs to be limited as early as possible by using artificial feed injected with exogenous enzymes (papain enzymes). This study aims to determine the dose of papain enzyme and the period of predigest feeding which results in the best growth and survival of barramundi larvae. Factorial pattern with completely randomized design was used to determine the appropriate dose of papain enzyme and larval age on growth and survival. The first factor was the dose of papain enzyme (0, 1.5, 3 and 4.5%), the second factor was the age of the larvae (14, 17 and 20 days).