Abstract: Paternity testing analysis aims to determine whether or not an allele parent is the biological parent of the child. If so, each allele of a child should match one allele of each parent. The most common technique used in paternity testing is the identification of short tandem repeat loci in order to evaluate the DNA evidence whether a man is not excluded by the paternal allele. In this case, a death victim was investigated. Two relatives, daughter and brother, were available for analysis. The VerifilerPlusTM PCR amplification kit was used for the investigation. We analyzed paternity testing of a child and putative father, by chance we found that a child and uncle shared 21 STRs loci. This study has shown a case which can be false inclusion if it were requested only a child and a relative of the biological father. The results suggest that for motherless paternity cases the use of 21 STR markers are not enough for investigation.