Abstract: Locke wrote his Letter Concerning Toleration in 1685, in which he argued for toleration of different religious views as the basis for a peaceful and civilized society. One of the main conceptions in this letter is ‘National Religion.’ Locke praises reformation in religion for human freedom and individual conscience. His tolerant philosophy is designed to establish moral authority for its ethical rules rather than show that they are most conducive to human nature. He thinks that reason is to determine the probability of the proposition on satisfactory evidence and a level of confidence in the proposal proportioned of likelihood. He considers that the articles of religion are practical and speculative, while moral actions required for a good life in civil society are available without direct faith. I argue that this article contains a new description, within the limits of my knowledge, to the concept of John Locke about ‘National Religion,’ therefore, it aims to submit a further reading to the religious perceptions represented by the central issue ‘National Religion’ in John Locke’s philosophy about his ideas on several issues, which linked with toleration, religious articles, human society, and civil interests.