Abstract: Sanitation is very important for human life. Safe sanitation is an essential component of health care practice and infection prevention and control strategies for preventing exposure of people to infections. This study aimed to determine knowledge of rural people on distribution of sanitation-related diseases in Ebonyi State, Nigeria. The study adopted a descriptive cross-sectional design to achieve the stated objective and the sample size was 461 obtained from primary source and it was collected from people interviewed with the questionnaire from the selected communities within the selected six (6) LGAs in the state. The statistical analysis was done with Statistical Package for Social Sciences (SPSS) version 20.0.