Abstract: To check the activity of nickel sulphate (0, 10, 20 and 30 mM) on radish seedlings through Foliar medium, whole soil culture experiment was performed in the wire house of old Botanical Garden, University of Agriculture Faisalabad. In this study it was noticed that nickle sulphate act as suppressor on growth of the plant and it also decreased the content of carotenoids and chlorophyll contents (Chlorophyll-a, b). Statistical analysis was achieved after collection of data by using appropriate computer software such as ANOVA. All morphological changes were observed to collect the data for the biochemical analysis. A marked reduction in chlorophyll a and chlorophyll b was noticed with slight elevation of carotenoid.