Abstract: Theileriosis in crossbred cattle is caused by Theileria annulata and is known as ‘Bovine Tropical Theileriosis’ in tropics. The chief vector responsible for transmission is Hyalomma anatolicum anatolicum and other ticks species belonging to the said genera. The disease is now reported more frequently and is of major economic importance due to losses associated with morbidity, mortality, decreased production as well as lowered working efficiency of affected animals. The present study was undertaken to evaluate hematological as well as biochemical alterations in crossbred cattle infected with Theileria annulata in Banaskantha district of Gujarat state, India.