Abstract: Fractionation of female and male spermatozoa has been practiced for selection of preferred sex of offspring to increase the profit in livestock industries. Furthermore, female calves are desired by the farmers for enhancing milk production in dairy cows, whereas in goats farmers prefer male calves than female calves for beef production. Meanwhile sexed semen for AI purpose in SL has been importing from India with high cost. Thus the objective of the present study is to separate ‘Y’ chromosome bearing sperm through low cost method for sustaining the male goat requirement satisfying the demands of male sex goat. Sucrose stock solution (2g/ml) was prepared. It was diluted with sodium citrate buffer solution to obtain the following densities such as 1g/ml, 1.1g/ml, 1.12g/ml and 1.13g/ml. The density gradient was prepared by pippetting 0.5ml of each sucrose solution along the wall of the tube and layered without mixing one another and then 10µl of diluted semen was dropped on the top of the layer. Then it was centrifuged (at 500 x g for 12 minutes at 24°C) for sexing the semen by means of density gradient and then each layer was examined to get the percentage of Y-bearing chromosome after eluting each layers. Statistical analysis was performed by using Prism 5.04. One way ANOVA with Bonferroni test was performed to compare the percentage of ‘Y’ chromosome in each sucrose gradient layer. The percentage of ‘Y’ chromosomes decreased significantly (P<0.05) for 1g/ml (59 ± 2.466, 62.56 ± 2.657%), 1.11g/ml (52.33 ± 0.8819, 56.75 ± 0.866 %), 1.12g/ml (41.00 ± 1.155, 45.34 ± 1.115 %) and 1.13g/ml (33.67 ± 0.8819, 43.9 ± 1.617) in Sannan and Jamunapari respectively. The present study revealed that this technique can be considered to establish low cost semen sexing in developing countries.