Abstract: Gujarat is a western state of India which enjoys a tropical climate. The major concentration of forests is found all along the eastern border of the state and the hilly portion of Saurashtra. The Jambughoda Wildlife Sanctuary lies towards the eastern part of the state between the parallels of latitude 22-22’N and 22-28’N and the meridians of longitude 73-38’E and 73-44’ E. The Sanctuary is spread over the Jambughoda and Halol Talukas of Panchmahals district and the Sankheda taluka of Vadodara district. The Sanctuary is spread over 130.38 Sq.Km. of undulating land with moist deciduous forest. This work deals with the analysis of soil samples collected from different areas of Jambughoda wildlife sanctuary. Stratified Random Sampling was employed to collect soil samples from surface as well as 10, 20 and 30 cm depths. The collected samples were analysed for macronutrients, micronutrients, organic carbon and organic matter. The amount of carbon sequestered was also calculated in kg/ha. Significant variations were seen in organic carbon content among samples collected from different places as well as from different depths. The average pH content of the soil samples was also measured.