Abstract: The experiment was conducted at the Laboratory of Agronomy Department, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during March to August 2013 to study the influence of variety and date of harvesting on changes of sugar and starch levels of potato derived from TPS at ambient storage condition. Potato variety and time of harvest had significant effect on nutritional aspects. Reducing sugar, total sugar increased with advancing storage period, whereas specific gravity, non-reducing sugar and starch content decreased with increasing storage period. Among the varieties HPS 364/67 was the best in respect of nutritional aspects. At harvest and 100 days after storage, the highest total sugar (0.61% and 0.82%) was observed from Lady Rosetta and harvest at 110 days after planting, while the lowest total sugar from HPS-364/67 and harvest at 80 days after planting. The results revealed that HPS 364/67 showed superior nutritional quality than other varieties when harvested at 100 days after planting.