Abstract: This research aims to: developing of biology learning instrument with guided inquiry model by life skill oriented in Madrasah Aliyah. The main purpose of this research to development of biology learning instrument with guided inquiry model by life skill oriented. This research was conducted in students at MA Amanatul Ummah Surabaya academic year 2018/2019. The sample in this research was 25 students of class X at MA Amanatul Ummah Surabaya academic year 2018/2019.